WebThis search provides access to all the entity’s information of record with the Secretary of State. For information on ordering certificates and/or copies of documents, refer to the … WebThe lysate is clarified by centrifugation at 5000 g and the nucleic acids precipitated by the addition of 7.5 ml 10% (v/v) polyethyleneimine per 100 ml lysate. The white precipitate is removed by centrifugation at 5000 g.

pCambia1300 Plant Expression Vector (ab275754) Abcam

WebNov 25, 2015 · 由此得出，CaMV35S启动PtrpC同样可oxysporum中起作用。 然而，本研究同样比较了带有PtrpCCaMV35SpCAMBIA一1300二者转差别很大，前者的转化效率可达到385-460个转化子／10绿木霉分生孢子，而后者转化效率仅为20-50转化子／10绿木霉分生孢子，仅为前者的1／10。 WebGeneral notes. Vector contains kanamycin resistance gene for bacterial selection, hygromycin B resistance gene for plant selection and no reporter gene to allow … cryptogamous meaning

农杆菌介导的绿木霉遗传转化及重寄生缺陷突变体的筛选 - 豆丁网

WebFeb 24, 2024 · pCAMBIA plant transformation vector. (A): an empty T-DNA construct hosting a neomycin phosphotransferase (nptII) gene driven by … WebNptII CaMV35S LacZ alpha CaMV35S CaMV35S polyA uidA Nos polyA LB RB Hind III NcoI AfIII Catalase intron Fig. 1 Schematic representation oftheT-DNAregionofthebinary vector pCambia 2301; LB left T-DNA border, RB right T-DNA border A new high-frequency transformation technique for S. indicum L. 1177. WebMar 8, 2016 · All these genes were driven individually by CaMV35S promoter and CaMV35S polyA terminator (Fig. 1a). The binary vector thus assembled was kindly provided by Dr. P. B. Kirti, University of Hyderabad, Hyderabad, India. The authenticity of the above construct was verified by sequencing of the cloned gene fragments. cryptogamic mats